Control strains for biochemical characteristics: Salmonella, E. coli, Proteus, Klebsiella, Citrobacter, Pseudomonas
on nutrient agar plates
Positive control for Mini-MSRV (artificial contamination): Salmonella
nutrient broth 10e9
MSRV (1plate/sample and 1 mini-plates/person) 2L
MKTTN (1 tube/sample) 1L
RVS (1 tube/sample) 1L
ONPG broth (6 tubes/group) 500ml
Lysine DC broth (6 tubes/group) 500ml
XLD (3 plates/sample) 2L (90 plates)
Rambach (3 plates/sample) 2L (90 plates)
Urea broth (6 tubes/group) 500ml (30 tubes)
Nutrient agar slopes (1 tube/person) 250ml (15 tubes)
XLD (MSR 48h)
Sven Gard agar blocked with corresponding antisera
Serotyping key, black paper, slides, Kaufmann-White scheme
Rest from Wednesday demonstration
09h00
Welcome and introduction by Pr. Tr?n Th? H?nh
Mini-MSRV: a quantitative method of Salmonella
detection
Interpretation of MSRV 24h
MSRV, Mini-MSRV 48h and inoculation on XLD
Sven Gard agar results (phase inversion), final serotyping and conclusions
09h15
General presentation of the training period, working and samplig plan
09h30
XLD and Rambach composition (presentation), transfer of MSRV, RVS and MKTTN on XLD and Rambach agar
09h45
Presentation of ISO 6579
10h00
Preparation of samples, BPW, stomacher, dilutions and mini-BPW for quantitative analysis
Transfer BPW to MSRV, MKTTN and RVS, Mini-BPW to Mini-MSRV
Interpretation of Mini-MSRV, inoculation of Urea broth, interpretation of Lysine, decarboxylase and ONPG tests, Urea/indole broth (presentation), inoculation of Urea broth and Urea/Indole broth
10h15
10h30
Presentation of API20E and inoculation
Links between the exercises of the week
10h45
11h00
11h15
11h30
Evaluations
12h00
LUNCH TIME
LUNCH TIME
LUNCH TIME
LUNCH TIME
CLOSING LUNCH
14h00
Introduction on Salmonella
and public health
ONPG and Lysine Decarboxylation tests, composition of media and principles
Principles of serotyping of Salmonella
strains (presentation)
Serotyping: agglutination and how to find oput a serotype? inoculation of Sven Gard plates for phase inversion
Forum and final restitution of P. Fravalo expertise: GLP, Cross-contamination, etc. Restitution of participants
14h30
Inoculation of ONPG and Lysine Dec broth, interpretation of Kliger results, interpretation of ONPG
15h00
Biochemical characteristics of Enterobacteriacea
How to calculate a sampling size? Practical sampling exercise
Inoculation of agar slope of a Salmonella
strain for serotyping
15h30
API20E results
16h00
Kliger inoculation with control strains ( Salmonella, E. coli, Proteus, Klebsiella, Citrobacter, Pseudomonas
)